SPOTO Giuseppe

Professore Ordinario

Orari di ricevimento

Tutti i giorni 9:30-12:30 15:30-17:30 previo appuntamento

Curriculum

Giuseppe Spoto

Professor of Analytical Chemistry
Dipartimento di Scienze Chimiche
Università degli Studi di Catania, Catania (Italy)

Address:
Viale Andrea Doria 6, 95125, Catania (Italy)
e-mail: gspoto@ unict.it

Education
Ph.D., University of Catania, 1996 (Chemistry)
“Laurea” Degree, University of Catania, 1992 (Chemistry)

Professional experience
-Scientific coordinator European Horizon 2020 project, ULTRAPLACAD (ULTRAsensitive PLAsmonic devices for early CAncer Diagnosis) May 2015- Oct. 2018. The consortium includes 13 partners from 7 European countries. Partners include 5 companies (Scriba Nanotechnologie , Italy; Ginolis, Finland; Future Diagnostics Solution, The Netherlands; Horiba Jobin Yvon, France; Amires, Czech Rep.)
-President of the Supervisory Committee Finanziamento Ricerca di Ateneo - FIR 2014 (University
Research funding) University of Catania. (2014)
-Member of the Board of Directors of Consorzio Catania Ricerche. Oct. 2014- 28th March 2017
-Management Committee Istituto Nazionale di Biostrutture e Biosistemi 2013- to date
-Member of the Steering Committee of Divisione Chimica Analitica (Italian Chemical Society) 2019-2021
-National Coordinator Gruppo Divisionale Spettroscopia Analitica (Italian Chemical Society) 2013- 2016.
-Management Committee substitute member European COST Action TD1003 Bio-inspired nanotechnologies: from concepts to applications 2011-2014.
-Head of Biomolecular Chemistry Degree Course (Laurea Magistrale) 2010- 2015.
-Member of the Steering Committee of the Gruppo Interdivisionale Spettroscopie Raman ed Effetti Ottici non Lineari (Italian Chemical Society) 2009-2012
-Member of the Steering Committee of Gruppo Divisionale Spettroscopia Analitica (Italian
Chemical Society) 2009-2012
-Full Professor of Analytical Chemistry, Università di Catania, 2005 – to date
-Research Associate, Institute of Biostructure and Bioimaging, Consiglio Nazionale delle Ricerche, 2004 - 2015
-Assistant Professor, Università di Catania, 2003 – 2005
-Visiting Researcher, Interface Analysis Centre, Bristol University (U.K.), Feb.-Aug.1994

Awards
Accademia Gioienia Award for Chemistry 1994
The Best Researcher Award, University of Catania, 2013

Grants

National
-Prin 2005, Unit responsible (Research and University Minister) (€ 72.000).
-Prin 2007, Unit resp. (Research and University Minister) (€ 61.000).
-Prin 2009, Unit resp.( Research and University Minister) (€ 32.000).
-HIPPOCRATES, PON02_00355, 2012-2015, Unit resp.INBB (Research and University Minister) (€ 359.000).
-Plast_ICs, PON02_00355_3416798, 2012-2015, Unit resp.INBB (Research and University Minister) (€ 282.000)
-PROFOOD, PON02_00451_3133441, 2012-2015, Unit resp.INBB (Research and University Minister) (€ 454.000).
-NANOMAX, 2013 (CNR) (€ 10.000).
-AHM fo CuHe (Advanced Green Materials for Cultural Heritage) (PNR 2015-2020, MIUR) 2018-2021 (€ 143.350)
-Prin 2017, Unit resp. (Research and University Minister) 2019-2022 (€ 194.332)

International

-ULTRAPLACAD (ULTRAsensitive PLAsmonic devices for early CAncer Diagnosis)
Project Coordinator (Horizon 2020 grant agreement n. 633937), 2015-2018 (European Commission, Bruxelles) (Total funding € 6.026.455. Unit € 833.487,50)
The consortium includes 13 partners from 7 countries. Partners include 5 European companies (Scriba Nanotechnologie , Italy; Ginolis, Finland; Future Diagnostics Solution, the Netherland; Horiba Jobin Yvon, France; Amires, Czech rep.).
ULTRAPLACAD proposal was ranked first among 462 proposals evaluated for the Horizon 2020 call PHC-10-2014 “Development of new diagnostic tools and technologies: in vitro devices, assays and platforms”.
The European Parliament included ULTRAPLACAD among the top 5 European research projects for cancer (https://www.what-europe-does-for-me.eu/en/portal/2/C02).

-AiPBAND (An Integrated Platform for Developing Brain Cancer Diagnostic Techniques) (H2020-MSCA-ITN-2017) Principal Investigator, 2018-2021 (Horizon 2020, European Commission, Bruxelles) (€ 258.061).

-VerSiLiB (Versatile Amplification Method for Single-Molecule Detection in Liquid Biopsy) (HORIZON-EIC-2021-PATHFINDEROPEN-01) Principal Investigator, 2022-2026 (Horizon Europe, European Commission, Bruxelles) (€ 495.410).

-DI-DIDA (Digital Innovations and Diagnostics for Infectious Diseases in Africa) (HORIZON-HLTH-2021-DISEASE-04-03) Principal Investigator, 2022-2027 (Horizon Europe, European Commission, Bruxelles) (€ 117.000).

Research grants from industry
-A.T.S. s.r.l., contratto di ricerca CNR, 2004 (€ 62.000).
-Neotron contratto di ricerca CNR, 2006 (€ 21.000).
-Meridionale Impianti S.p.A., contratto di ricerca, 2006 (€ 230.000).
-Dipietrogroup s.r.l. contratto di ricerca, 2012 (€ 120.000).

Review of research proposals
-MIUR (PRIN, VQR, Futuro in Ricerca, Programma per Giovani Ricercatori "Rita Levi Montalcini") (Italy)
- The Netherlands Foundation for Fundamental Research on Matter (FOM), Olanda.
- Austrian Institute of Technology (Austria)
- Research and Innovation SERI (Swiss Confederation)
- Deutsche Forschungsgemeinschaft (German Research Foundation)
- Dutch National Science Foundation (The Netherland)
- Foundation for Polish Science (Polonia)
- European Commission (FET Open – project monitor)
- European Commission (ERC Consolidator Grant)

Invited lectures
V Seminario Nazionale di Spettroscopia Analitica, Gargnano, 2001.
IV Scuola Nazionale di Chimica per i Beni Culturali. Vetro e Ceramica, Altare (SV), 2002. University of Lecce, 15 November 2002, Lecce.
University of Parma, 21 September 2004, Parma
Scuola Nazionale di Chimica Analitica per Dottorandi, 26 September 2006, Rome LASERION 2007, 1-6 July 2007 Tegernsee, (Germany).
Nano-Bio-Photonics: Converging Science and Technologies Status and Perspectives, 14 September 2007, Rome.
Biosensori per l’ambiente e per la salute, 9-10 October 2007, Rome,.
First BioInterface School, 16 Novembre 2007, Lecce.
SCI Convegno Congiunto sezioni Calabria e Sicilia, 4- Dicembre 2007, Messina.
European Materials Research Society Spring Meeting, Strasbourg (France), 2009.
CNR, Bologna, 15 June 2010.
LASERION 2010, 7-10 July 2010 Tegernsee, (Germany)
TECHNART 2011, 26 29 April 2011 Berlin, (Germany) .
NANOMED Italy-Sweden Bilateral Meeting, 29-30 Settember, Stockholm, (Sweden).
University of Torino, 21 October 2011, Torino.
Advanced laser-based techniques in art conservation, diagnostics and analysis, FORTH,
Heraklion, 22 June 2012, (Greece)
Department of Chemistry, University of Crete, 23 June 2012, (Greece).
XXIII National Congress of the Analytical Chemistry Division Italian Chemical Society, 20
September 2012 (Keynote Lecture)
Micro and Nanosystems in Biochemical Analysis, 17-20 October 2012, Warsaw, (Poland) Bioelectrochemistry 2013, 17-21 March 2013, Bochum, (Germany) Laserion 2013, 3-6 July 2013, Tegernsee, (Germany).
4th Summer Symposium on Nanomaterials and their application to Biology and Medicine, 15th18th June 2014, Poznań, (Poland)
CEITEC Annual Conference on “Frontiers in material and life sciences“, 21th – 24th October 2014, Brno, (Czech Republic).
3rd International Conference BioPhotonics 2015, 20th-22nd May 2015, Florence, Italy
5th Summer Symposium on Nanomaterials and their application to Biology and Medicine, 14th18th June 2015, Zakopane, (Poland).
GS2015, 15th-17th June 2015, Parma, Italy
Orbitaly 2015, 10-11 September 2015, Modena, Italy.
AIT- Austrian Institute of Technology, 30th June 2016, Vienna, Austria.
Nano and Photonics 2017, 24 March 2017, Mauterndorf, Austria.
TRACE workshop, 29 June 2017, Dublin, Ireland.
Department of Chemistry “Ugo Schiff", University of Florence, 6 September 2017, (Italy)
Winter School on Biophotonics and Bioelectronics, 22 February 2018, Hirshegg, Austria.
Twente University, Mesa+, 17 May 2018, Enschede, The Netherlands.
Bioelectrochemistry, Surfaces, and more… 2019, 6 February 2019, Tulln, Austria.
MADIA workshop.11-12 June 2019. Bologna
PIERS 2019, PhotonIcs & Electromagnetics Research Symposium, 17-20 June 2019, Rome.
Workshop “From Molecules to Devices”, 6 December 2019, Parma.
European Conference on Novel Technologies for In Vitro Diagnostics – DIATECH 2020, 26-29 January 2020, Leuven, Belgium (Keynote).
Biomedical Engineering Division, University of Glasgow. Online meeting. 24 March 2021
NANOFACTS training school, BioSense Institute, University of Novi Sad, Serbia. 27th June 2022.

Research activities
Biosensors and microfluidics.
Advanced in vitro diagnostic methods for clinical applications
Molecular and biomolecular systems at the solid/liquid interface

Publications
He has authored 109 papers that have been published in peer-reviewed international journals and in book chapters.

Books
-Modern Analytical Methods in Art and Archaeology, E. Ciliberto, G. Spoto, Eds., John Wiley & Sons, Inc., New York, 2000.
-Detection of Non-Amplified Genomic DNA, G. Spoto, R. Corradini, Eds., Springer, 2012.

Papers (2005 - 2022)
37. E. De Giglio, S. Cometa, L. Sabbatini, P.G. Zambonin, G. Spoto,
“Electrosynthesis and analytical characterization of PMMA coatings on titanium substrates as barrier against ion release”, Analytical and Bioanalytical Chemistry, Springer-Verlag Heidelberg, Gennaio 2005, 381(3), 626 - 633.

38. G. Arena, R.. P. Bonomo, A. Contino, C. Sgarlata, G. Spoto, G. Tabbì
“Influence of the coordination geometry on the physicochemical properties of a copper(II) complex with a tailor made calixarene-based ligand bearing dipyridyl pendants. An ESR, UV-Vis and CV study” Dalton Transactions, The Royal Society of Chemistry, London (U.K.), 2004, (20), 3205 – 3211.

39 G. Arena, A. Contino, R. D’Agata, C. Sgarlata, G. Spoto
“Ordered anchored cavities at work: A new and rapid SPR-based method for the detection of trace amounts of Cs+”. New Journal of Chemistry, The Royal Society of Chemistry, London (U.K.), Novembre, 2005, 29, 1393 - 1395.

40. G. Grasso, R. D’Agata, E. Rizzarelli, G. Spoto, L. D'Andrea, C. Pedone, A. Picardi, A. Romanelli, M. Fragai, K. Joo Yeo *
“Activity of Anchored Human Matrix Metalloproteinase-1 Catalytic Domain on Au (111) Surfaces Monitored by ESI-MS”. Journal of Mass Spectrometry, Wiley Interscience, Dicembre, 2005, 40, 1565-1571.

41. R. D’Agata, G. Grasso, G. Iacono, G. Spoto, G. Vecchio
“A Surface Plasmon Resonance Imaging study of the lectin recognition of a new SOD mimic bioconjugate”. Organic & Biomolecular Chemistry, The Royal Society of Chemistry, London (U.K.), Febbraio, 2006, 4; 610-612.

42 G. Spoto,
“Chemical Methods in Archaeology” Kirk-Othmer Encyclopedia of Chemical Technology, Concise, 5th Edition, (ISBN 978-0-470-04748-4), John Wiley & Sons Inc., New York., (USA) Maggio 2007, 73-75.

43 G. Grasso, M. Fragai, E. Rizzarelli, G. Spoto, K.J. Yeo,
“In Situ AP/MALDI-MS characterization of anchored MMPs”, Journal of Mass Spectrometry, Wiley Interscience, Dicembre 2006; 41: 1561–1569

44 G. Grasso, M. Fragai, E. Rizzarelli, G. Spoto, K.J. Yeo,
“A new methodology for monitoring the activity of cdMMP-12 anchored and freeze-dried on Au (111)”, Journal of the American Society for Mass Spectrometry, Maggio 2007, 18(5), 961-969.

45 G. Arena, I. Deretzis, G. Forte, F. Giannazzo, A. La Magna, G. Lombardo, V. Raineri, C. Sgarlata, G. Spoto
“Electron transport properties of calix[4]arene based systems in a metal-molecule-metal junction: characterization by conducting-atomic force microscopy and theoretical calculations”, New Journal of Chemistry, The Royal Society of Chemistry, London (U.K.), 2007, 31(5), 756-761.

46 R. D’Agata, G. Grasso, S. Parlato, S. Simone, G. Spoto,
“The Use of Atmospheric Pressure Laser Desorption Mass Spectrometry for the Study of Iron-Gall Ink”, Applied Physics A, Springer, Berlin (Germania). 2007, 89, 91-95. Invited paper

47 . G. Grasso, E. Rizzarelli, G. Spoto
AP/MALDI-MS complete characterization of Insulin fragments produced by the interaction of IDE with bovine Insulin, Journal of Mass Spectrometry Wiley Interscience, Dicembre 2007, 42, 1590-1598.

48. M. Licciardello, R. D’Agata, G. Grasso, S. Simone, G. Spoto,
Atmospheric Pressure Laser Desorption Mass Spectrometry based methods for the study of traditional painting materials, in Laser in Conservation of Artworks, (Ed. M. Castillejo), Taylor & Francis, London, 2008, 67-72.

49. R. D’Agata, G. Grasso, G. Spoto
Real-time binding kinetics monitored with surface plasmon resonance imaging in a diffusion-free environment, The Open Spectroscopy Journal, Bentham Science Publishers, 2008, 1, 1-9.

50. G. Grasso, E. Rizzarelli, G. Spoto
How the binding and degrading capabilities of insulin degrading enzyme are affected by ubiquitin, Biochimica et Biophysica Acta - Proteins and Proteomics, Elsevier, 2008, 1784, 1122-1126.

51. R. D’Agata, R. Corradini, G. Grasso, R. Marchelli, G. Spoto
Ultrasensitive Detection of DNA by PNA and Nanoparticle-Enhanced Surface Plasmon Resonance Imaging, ChemBioChem, Wiley Interscience, Agosto, 2008, 9(13), 2067-2070

52. Giuseppe Grasso,Ashley I. Bush, Roberta D’Agata, Enrico Rizzarelli, Giuseppe Spoto
Enzyme solid-state support assays: a surface plasmon resonance and mass spectrometry coupled study of immobilized insulin degrading enzyme, EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS, Springer, 2009, 38(4), 407-414.

53. Giuseppe Grasso, Enrico Rizzarelli, Giuseppe Spoto
The Proteolytic Activity of Insulin-Degrading Enzyme: a Mass Spectrometry Study
Journal of Mass Spectrometry Wiley Interscience, 2009, 44(5), 735-741.

54. M. Coletta; C. Ciaccio, G. R Tundo, G. Grasso, G. Spoto, D. Marasco, M. Ruvo, M. Gioia, E. Rizzarelli, “Somatostatin: a novel substrate and a modulator of insulin-degrading enzyme activity” Journal of Molecular Biology, Elsevier, (2009) 385, 1556–1567

55. R. D’Agata, R.Corradini, R. Marchelli, G. Spoto
Surface plasmon resonance imaging e acidi peptido nucleici: un approccio combinato per la rivelazione ultrasensibile di DNA, La Chimica & l’Industria, (2009), 5, 92-97.

56. L. Zanoli, R. D’Agata, G. Spoto
“Surface Plasmon-based Optical Detection of DNA by Peptide Nucleic Acids”
Minerva Biotecnologica: Journal on Biotechnology and Molecular Biology, Minerva Medica, (2008), 20, 165-174.

57. Rosangela Marchelli, Emanuela Licandro, Michele Saviano, Giuseppe Spoto, Monica Borgatti Roberto Gambari
“PNAs targeting the “RNA world”: micro RNA next?”
Minerva Biotecnologica: Journal on Biotechnology and Molecular Biology, Minerva Medica, (2008), 20, 183-188.

58 G. Grasso, P. Mineo, E. Rizzarelli, G. Spoto
“MALDI, AP/MALDI and ESI techniques for the MS detection of amyloid-β peptides”, International Journal of Mass Spectrometry, Elsevier, (2009), 282(1-2), 50-55.

59. G. Grasso, R. D’Agata, L. Zanoli, G. Spoto
“Microfluidic Networks for Surface Plasmon Resonance Imaging Real-Time Kinetics
Experiments”, Microchemical Journal, Elsevier, (2009), 93, 82–86.

60. G. Grasso, L. D’Urso, E. Messina, F. Cataldo,O. Puglisi, G. Spoto, G. Compagnini
“A mass spectrometry and surface enhanced Raman spectroscopy study of the interaction between linear carbon chains and noble metals”, Carbon, (2009), 47(11), Elsevier, 2611-2619.

61. L. Giurato, A. Candura, G. Grasso, G. Spoto *
In Situ Identification of Organic Components of Ink Used in Books from the 1900s by Atmospheric Pressure Matrix Assisted Laser Desorption Ionization Mass Spectrometry ”, Applied Physics A, Springer, (2009), 97, 263-269. Invited paper

62. L. D'Urso, G. Grasso, E. Messina, C. Bongiorno, V. Scuderi, S. Scalese, O. Puglisi, G. Spoto, G. Compagnini,
"The Role of Linear Carbon Chains on the Aggregation of Copper, Silver and Gold Nanoparticles" The Journal of Physical Chemistry C, (2010), 114, 907–915.

63. G. Spoto,
“Spatially Resolved MS in the Study of Art and Archaeological Objects” in “Handbook of Mass Spectrometry”, First Edition. Ed. Mike S. Lee, John Wiley & Sons, New York, 2012.

64. R. D’Agata, R. Corradini, C. Ferretti, L. Zanoli, M. Gatti, R. Marchelli, G. Spoto *
“Ultrasensitive detection of non-amplified genomic DNA by nanoparticle-enhanced Surface-Plasmon Resonance Imaging”, Biosensors & Bioelectronics, Elsevier, (2010), 25, 2095–2100.

65. R. D’Agata, R. Corradini, C. Ferretti, L. Zanoli, M. Gatti, R. Marchelli, G. Spoto *
“Detection of non amplified genomic DNA by advanced SPRI methods”, La Chimica & l’Industria, (2010), 7, 140-145.

66. D' Agata R, Breveglieri G., Zanoli L, Borgatti M, Spoto G, Gambari R *
Surface Plasmon Resonance Imaging (SPR-I), peptide nucleic acid (PNA) probes and nanoparticle-enhancement for PCR-free ultrasensitive detection of beta-thalassemia mutations in human genomic DNA. International Journal of Molecular Medicine, (2010), 26; S61 ISSN: 1107-3756.

67. Giuseppe Grasso, Adriana Pietropaolo, Giuseppe Spoto, Giuseppe Pappalardo, Grazia Raffaella Tundo, Chiara Ciaccio, Massimo Coletta, Enrico Rizzarelli
“Copper(I) and copper(II) inhibit Aβ peptides proteolysis by insulin-degrading enzyme differently: implications for metallostasis alteration in Alzheimer’s disease”, Chemistry – A European Journal, (2011), 17(9), 2752–2762.

68. Laura Maria Zanoli, Roberta D’agata, Giuseppe Spoto, Roberto Corradini, Rosangela Marchelli, Cristina Ferretti, Marcello Gatti *
“Ultrasensitive Detection of Non Amplified Genomic DNA” in Sensors and Microsystems, Eds. G. Neri, N. Donato, A. D'Amico, C. Di Natale, Springer, 2011, 485-493.

69. G. Spoto, G. Grasso *
“Spatially Resolved Mass Spectrometry: Applications in the Study of Art and Archaeological Objects”, TRAC – Trends in Analytical Chemistry, (2011), 30(6), 856-863.

70. R. D'Agata, L.M. Zanoli, G. Breveglieri, M. Borgatti, A. Finotti, G. Spoto, R. Gambari, *
“Detection of genomic disorders in unamplified human genomic DNA using an ultrasensitive surface plasmon resonance imaging method” International Journal of Molecular Medicine (2011), 28, S59.
71. L. M. Zanoli, R. D’Agata, G. Spoto *
“Functionalized gold nanoparticles for the ultrasensitive DNA detection” Analytical and Bioanalytical Chemistry, Springer-Verlag, (2012) 402, 1759–1771. (Selected Hot Article)

72. Nevin, G. Spoto, D. Anglos *
“Laser spectroscopies for elemental and molecular analysis in art and archaeology” Applied Physics A, Springer, (2012) 106, 339–361.

73. Roberta D'Agata , Giulia Breveglieri , Laura Maria Zanoli , Monica Borgatti, Giuseppe Spoto, Roberto Gambari *
“Direct Detection of Point Mutations in Non-amplified Human Genomic DNA” Analytical Chemistry. American Chemical Society, (2011) 83(22), 8711-8717.

74. Laura Maria Zanoli, Marco Licciardello, Roberta D’Agata, Claudia Lantano, Alessandro Calabretta, Roberto Corradini, Rosangela Marchelli, Giuseppe Spoto *
“Peptide Nucleic Acid Molecular Beacons for the Detection of PCR Amplicons in Droplet-Based Microfluidic Devices” Analytical and Bioanalytical Chemistry, Springer-Verlag, (2013), 405 (2-3) 615-624. DOI: 10.1007/s00216-011-5638-3.
75. R. D’Agata, G. Spoto,
“Surface plasmon resonance-based methods”, in Detection of Non Amplified Genomic DNA, Eds. R. Corradini, G. Spoto, Springer-Verlag, 2012 (ISBN 978-94-007-1225-6).
76. R. D’Agata, G. Spoto
“Artificial DNAs and surface plasmon resonance” Artificial DNA: PNA & XNA, Landes Biosciences, 2012, 3(2), 45-52.
77. Giuseppe Grasso, Fabrizio Salomone, Grazia R. Tundo, Giuseppe Pappalardo, Chiara Ciaccio, Giuseppe Spoto, Adriana Pietropaolo, Massimo Coletta, Enrico Rizzarelli
Metal ions affect insulin-degrading enzyme activity Journal of Inorganic Biochemistry (2012) 117, 351-358, doi: 10.1016/j.jinorgbio.2012.06.010
78. L.M. Zanoli, G. Spoto
Isothermal Amplification Methods for the Detection of Nucleic Acids in Microfluidic Devices Biosensors 2013, 3, 18-43; doi:10.3390/bios3010018
79. G. Grasso, G. Spoto
Plasmonics for the study of metal ion-protein interactions Analytical and Bioanalytical Chemistry, 10.1007/s00216-012-6421-9. 2013 Feb;405(6):1833-43

80. G. Spoto, M. Minunni
“Surface Plasmon Resonance imaging: What's next?”, The Journal of Physical Chemistry Letters, American Chemical Society, 2012, 3, 2682−2691

81. R. D’Agata, G. Spoto
“Surface Plasmon Resonance Imaging for Nucleic Acid Detection” Analytical and Bioanalytical Chemistry, 2013, 405 (2-3), 573-584.
82. G. Spoto
“Chemical Methods in Archaeology”, Kirk-Othmer Encyclopedia of Chemical Technology, John Wiley & Sons, Inc., 2013, DOI: 10.1002/0471238961.0308051319161520.a01.pub2
83 V. Oliveri, R. D'Agata, V. Giglio, G. Spoto, G. Vecchio
“Cyclodextrin-functionalised gold nanoparticles via streptavidin: a supramolecular approach” Supramolecular Chemistry, 25(8), 2013, 465-473

84 A. Bertucci, A. Manicardi, A. Candiani, S. Giannetti, A. Cucinotta, G. Spoto, M. Konstantaki, S. Pissadakis, S. Selleri, R. Corradini
“Detection of unamplified genomic DNA by a PNA-based microstructured optical fiber (MOF) Bragg-grating optofluidic system”. Biosensors and Bioelectronics 63 (2015) 248–254.

85. Maria Chiara Giuffrida, Laura Maria Zanoli, Roberta D’Agata, Alessia Finotti, Roberto Gambari, Giuseppe Spoto
“Isothermal circular strand displacement polymerization of DNA and microRNA in digital microfluidic devices”, Analytical & Bioanalytical Chemistry 407 (2015) 1533–1543. special issue “Rising Stars and Top Experts”

86 Valeria Lanza, Roberta D'Agata, Giuseppe Iacono, Francesco Bellia, Giuseppe Spoto, Graziella Vecchio
Cyclam glycoconjugates as lectin ligands and protective agents of metal-induced amyloid aggregation Journal of Inorganic Biochemistry 153 (2015) 377–382

87. M. C. Giuffrida, L. M. Zanoli, R. D’Agata, A. Finotti, J. Gasparello, M. Borgatti, R. Gambari, G. Spoto.
Digital microfluidic devices for isothermal circular strand displacement polymerization of microRNAs. International Journal of Molecular Medicine, Vol. 36, Suppl 1, 2015, pag. S106.

88. P. Palladino. A.M. Aura, G. Spoto
Surface Plasmon Resonance For The Label-Free Detection of Alzheimer’s β-Amyloid Peptide Aggregation. Analytical & Bioanalytical Chemistry (2016) 408 (3), 849-854. DOI: 10.1007/s00216-015-9172-6.

89. Valentina Giglio, Maurizio Viale, Massimiliano Monticone, Angela Margherita Aura, Giuseppe Spoto, Giovanni Natile, Francesco Paolo Intini, Graziella Vecchio.
Cyclodextrin polymers as carries for the platinum-based anticancer agent LA-12. RSC Advances. (2016) 6, 12461-12466.

90. N. Bellassai, G. Spoto
Biosensors for liquid biopsy: circulating nucleic acids to diagnose and treat cancer. Analytical & Bioanalytical Chemistry (2016) 408:7255-7264. DOI 10.1007/s00216-016-9806-3.

91 M.C. Giuffrida, G. Spoto
Integration of isothermal amplification methods in microfluidic devices: recent advances. Biosensors and Bioelectronics. 90 (2017) 174–186. 10.1016/j.bios.2016.11.045

92. R. D’Agata, P. Palladino, G. Spoto
Streptavidin-coated gold nanoparticles: critical role of oligonucleotides on stability and fractal aggregation. Beilstein Journal of Nanotechnology, 2017, 8, 1-11.

93. M. C. Giuffrida, R. D’Agata, G. Spoto
Droplet Microfluidic Device Fabrication and Use for Isothermal Amplification and Detection of MicroRNA. In MicroRNA Detection and Target Identification: Methods and Protocols, Methods in Molecular Biology, Tamas Dalmay (ed.), vol. 1580, DOI 10.1007/978-1-4939-6866-4_6, Springer Science+Business Media LLC, 2017, pp. 71-78.

94. G. Grasso, M. Calcagno, A. Rapisarda, R. D’Agata, G Spoto
Atmospheric pressure MALDI for the non-invasive characterization of carbonaceous ink from Renaissance documents. Analytical & Bioanalytical Chemistry. 2017, 409:3943–3950.

95 AM Aura, R D'Agata, G Spoto
Ultrasensitive Detection of Staphylococcus aureus and Listeria monocytogenes Genomic DNA by Nanoparticle-Enhanced Surface Plasmon Resonance Imaging. ChemistrySelect 2 (24), 2017, 7024–7030

96 R D'Agata, MC Giuffrida, G Spoto
Peptide Nucleic Acid-Based Biosensors for Cancer Diagnosis. Molecules 22 (11), 2017, 1951

97. Marcello Berto, Chiara Diacci, Roberta D’Agata, Marcello Pinti, Elena Bianchini, Michele Di Lauro, Stefano Casalini, Andrea Cossarizza, Magnus Berggren, Daniel Simon, Giuseppe Spoto, Fabio Biscarini, Carlo A. Bortolotti
EGOFET Peptide Aptasensor for Label-Free Detection of Inflammatory Cytokines in Complex Fluids. Advanced Biosystems, 2017, 1700072

98 Maria Chiara Giuffrida, Giovanni Cigliana, Giuseppe Spoto,
Ultrasensitive detection of lysozyme in droplet-based microfluidic devices. Biosensors and Bioelectronics, 104, 2018, 8-14. doi: 10.1016/j.bios.2017.12.042

99 A. Finotti, M. Allegretti, J. Gasparello, P. Giacomini, D.A. Spandidos, Giuseppe Spoto, R. Gambari,
Liquid biopsy and PCR-free ultrasensitive detection systems in oncology. International Journal of Oncology, 53(4), 2018, 1395-1434.

100 Noemi Bellassai, Almudena Marti, Giuseppe Spoto, Jurriaan Huskens
Low-fouling, mixed-charge poly-L-lysine polymers with anionic oligopeptide side-chains. Journal of Materials Chemistry B, 2018, 6, 7662. DOI:10.1039/C8TB01619D

101 R D'Agata, G Spoto, Advanced methods for microRNA biosensing: a problem-solving perspective. Analytical and Bioanalytical Chemistry, 2019. doi.org/10.1007/s00216-019-01621

102 G. Spoto, Mass Spectrometry in the Study of Art and Archaeological Objects, in Mass Spectrometry: An Applied Approach. edited by Marek Smoluch, Giuseppe Grasso, Piotr Suder, Jerzy Silberring. Wiley, 2019, 345-350

103 Noemi Bellassai, Roberta D'Agata, Vanessa Jungbluth, Giuseppe Spoto, Surface Plasmon Resonance for Biomarker Detection: Advances in Non-invasive Cancer Diagnosis. Frontiers in Chemistry, 7, 2019, 570. DOI: 10.3389/fchem.2019.00570.

104 D'Agata, R., Bellassai, N., Allegretti, M., Rozzi, A., Korom, S., Manicardi, A., Melucci, E., Pescarmona, E., Corradini, R., Giacomini, P., Spoto, G. Direct plasmonic detection of circulating RAS mutated DNA in colorectal cancer patients. Biosensors and Bioelectronics, 170, 15 December 2020, 112648

105 D'Agata, R., Bellassai, N., Giuffrida, M.C., Aura, A.M., Petri, C., Kögler, P., Vecchio, G., Jonas, U., Spoto, G. A new ultralow fouling surface for the analysis of human plasma samples with surface plasmon resonance. Talanta, 221, 1 January 2021, 121483

106 Meenu Selvaraj, Pierpaolo Greco, Matteo Sensi, Gulseren Deniz Saygin, Noemi Bellassai, Roberta D’Agata, Giuseppe Spoto, Fabio Biscarini. Label free detection of miRNA-21 with Electrolyte Gated Organic Field Effect Transistors (EGOFETs). Biosensors and Bioelectronics, 182, 2021, 113144.

107. Noemi Bellassai, Roberta D’Agata, Giuseppe Spoto. Novel nucleic acid origami structures and conventional molecular beacon-based platforms: a comparison in biosensing applications. Analytical and Bioanalytical Chemistry, 2021, March 2021, https://doi.org/10.1007/s00216-021-03309-4.

108. Noemi Bellassai, Roberta D’Agata, Almudena Marti, Andrea Rozzi, Stefano Volpi, Matteo Allegretti, Roberto Corradini, Patrizio Giacomini, Jurriaan Huskens, Giuseppe Spoto. Detection of Tumor DNA in Human Plasma with a Functional PLL-Based Surface Layer and Plasmonic Biosensing. ACS Sensors. 2021. 6(6), 2307–2319 https://doi.org/10.1021/acssensors.1c00360

109. Roberta D’Agata, Noemi Bellassai, Vanessa Jungbluth, Giuseppe Spoto. Recent Advances in Antifouling Materials for Surface Plasmon Resonance Biosensing in Clinical Diagnostics and Food Safety. Polymers 2021, 13(12), 1929; https://doi.org/10.3390/polym13121929

110. Marzia Calcagno, Roberta D’Agata, Giulia Breveglieri, Monica Borgatti, Noemi Bellassai, Roberto Gambari, Giuseppe Spoto. Nanoparticle-Enhanced Surface Plasmon Resonance Imaging Enables the Ultrasensitive Detection of Non-Amplified Cell-Free Fetal DNA for Non-Invasive Prenatal Testing. Analytical Chemistry 2022, 94, 2, 1118–1125. https://doi.org/10.1021/acs.analchem.1c04196

111 Noemi Bellassai, Roberta D’Agata, Giuseppe Spoto, Isothermal circular strand displacement–based assay for microRNA detection in liquid biopsy. Analytical and Bioanalytical Chemistry 2022, https://doi.org/10.1007/s00216-022-04228